Quantitative proteome of acquired enamel pellicle of healthy versus periodontal disease patients

PLEASE NOTE: This work is protected by copyright. Downloading is restricted to the BU community: please click Download and log in with a valid BU account to access. If you are the author of this work and would like to make it publicly available, please contact open-help@bu.edu.Thesis (MSD) --Boston University, Goldman School of Dental Medicine, 2012 (Department of Periodontology and Oral Biology).Includes bibliographic references: leaves 108-117.Oral health is one of the important areas of the whole human body anatomy. So many disease symptoms can reveal their development at one level or another in the oral cavity. There have been many studies carried out in this area. Scientists try to improve the Oral Health and prevent disease onset. Periodontal diseases and the Caries are the most common problem related to oral health. These problems can be directly or indirectly related to acquired enamel pellicle (AEP). This is because AEP is formed on the enamel surface and can influence occurrence of both periodontal disease as well as caries. In this study our aim was to investigate in detail AEP composition differences between the health individuals and periodontal disease patients. Periodontal diseases are a bacterial infection associated with a chronic inflammatory response which effects tooth supporting structures. They are result of gingival inflammation, alveolar bone loss, tooth mobility and in the severe cases tooth loss. In addition they change the serum related components in the oral cavity via GCF (Gingival Cervical Fluid) and new components from the inflammatory reaction and bone resorption. These changes can be used to monitor the onset, severity, and progression of the disorder. Therefore the determination of salivary biomarkers has been an essential role of monitoring general health and for the early diagnosis of diseases. In order to identify these of oral fluids and protein components such as AEP, GCF and saliva to compare the differences between oral health and disease by using special laboratory techniques. The present study coupled mass spectrometry (MS) with amine-specific Tag for Relative and Absolute Quantitation (mTRAQ) methods for the identification and relative quantification of AEP proteins of patients with moderate to severe periodontal disease and compared the results with samples from the healthy group. This permitted a large scale proteomic screening approaches for proteins that have the potential as diagnostic biomarkers for periodontal disease. 144 distinct AEP proteins were identified and quantified in this study, of which 43 were significantly elevated in samples from patients with periodontal disease