Vaccine

BackgroundSeasonal influenza vaccines aim to induce strain-specific neutralizing antibodies. Non-neutralizing antibodies may be more broadly cross-reactive and still protect through mechanisms including antibody-dependent cell-mediated cytotoxicity (ADCC). Influenza vaccines may stimulate ADCC antibodies in adults, but whether they do so in children is unknown. Here we examined how vaccination affects cross-reactive ADCC antibody responses in children after receipt of inactivated trivalent vaccine (IIV3) or quadrivalent live-attenuated vaccine (LAIV4).MethodsChildren aged 5\u201317 were recruited in fall 2014 to provide pre- and post-vaccination serum samples. Children aged 5\u20139 received LAIV4 based on then-current recommendation, and older children were randomly assigned to IIV3 or LAIV4. We used microtiter-plate-based flow cytometry with an NK cell line to examine ADCC antibody responses to the 2014\u201315 H3N2 vaccine component (A/Texas/50/2012 [TX12]) and a drifted strain, A/Switzerland/9715293/2013 (SW13). Responses were stratified by two-season (2013\u201314 and 2014\u201315) vaccine sequence.ResultsEighty-five children received LAIV4 and 45 received IIV3. Prevaccination ADCC activity was highest in children who had received any vaccine in the prior season. Increase in ADCC antibody responses against the vaccine strain TX12 following vaccination was greatest for participants who received IIV3 in 2014\u201315 and LAIV4 in the prior season (geometric mean fold rise [MFR]=1.6, 95% CI 1.23 \u2013 2.11). This group also had a detectable ADCC response to the drifted SW13 strain. There was a modest ADCC response against SW13 in LAIV4 recipients who were unvaccinated in the previous season (MFR=1.18, 95% CI 1.10 \u2013 1.25). There were no significant changes in 2014\u201315 ADCC response to vaccination among children who had received IIV3 in 2013\u201314.ConclusionsVaccinating children with IIV3 after prior receipt of LAIV4 generated a modest increase in ADCC antibodies, including some cross-reactivity with an emerging drift variant. Other vaccine-induced ADCC responses were minimal and not affected by vaccine type or sequence.20192021-02-18T00:00:00ZU01 IP000471/IP/NCIRD CDC HHS/United StatesU01 IP001038/IP/NCIRD CDC HHS/United States31753674PMC7028493762