The analysis of reproduction organs and sperm quality of culled stock bulls

The aim of this work was to analyse the reproduction organs and sperm quality from the 16 culled stock bulls: perform the research of frozen, fresh semen, and the semen taken from (research the mobility, concentration, pathological spermatoza of semen). The analysis took place in 2018–2019 LSMU VA Animal reproduction laboratory and in LSMU VA pathology centre – necropsy hall. In total we had 32 bull testis (left and right), 8 samples of fresh semen (4 – from the first ejaculation and 4 from the second ejaculation); 16 samples of frozen semen and 12 samples of semen taken from the epididymis, at total we had 36 samples of semen. During the research we did the measurments of testis and epididymis, we searched for any microscopic and macroscopic pathology. We have compared the sizes of testis with the results of semen analysis, researched how one value conditions another value. During the frozen sperm research we found out that the average mobility of sperm was 61.5 ± 12.6 percent. Only one of sample of frozen semen did not match the minimum norm, that is set in the general requirements of bull semen (normal > 40 %). The sperm mobility of the fresh semen sample from the first ejaculation was 83.5 ± 9 percent, second ejaculations mobility was 79.5 ± 16 percent. The morfological semen research showed us that all the samples of semen matches the minimum requirements of bull semen. The average pathological sperm in the fresh semen sample was 8.25 ± 2.5 % from the first ejaculation, and from the second ejaculation – 7.7 ± 2.2 %, and on the frozen semen samples we found that the average count of pathological sperm was 4.7 ± 1.7 %