Dibucaine in Ionic-Gradient Liposomes: Biophysical, Toxicological, and Activity Characterization
- Couto, Verônica M.
- Prieto, Maria Jimena
- Igartúa, Daniela
- Feas, Daniela Agustina
- Ribeiro, Lígia N.M.
- Silva, Camila M.G.
- Castro, Simone R.
- Guilherme, Viviane A.
- Dantzger, Darlene D.
- Machado, Daisy
- Alonso, Silvia del Valle
- de Paula, Eneida
DOIAbstract
Administration of local anesthetics is one of the most effective pain control techniques for postoperative analgesia. However, anesthetic agents easily diffuse into the injection site, limiting the time of anesthesia. One approach to prolong analgesia is to entrap local anesthetic agents in nanostructured carriers (e.g., liposomes). Here, we report that using an ammonium sulphate gradient was the best strategy to improve the encapsulation (62.6%) of dibucaine (DBC) into liposomes. Light scattering and nanotracking analyses were used to characterize vesicle properties, such as, size, polydispersity, zeta potentials, and number. In vitro kinetic experiments revealed the sustained release of DBC (50% in 7 h) from the liposomes. In addition, in...
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