Verification of hypochromic erythrocytes on Sysmex XN 2000

Anemia is a serious blood disease that more than two billion suffer from worldwide. Half of that number consist of iron deficiency anemia, which is insufficiency of iron supply to the bone marrow due to empty iron store or hampered iron utilization due to inflammation. Therefore, requires rapid diagnosis. Analysis parameter for hypochromic erythrocytes (HYPO-He) is a highly applicable parameter for detection of iron deficiency using Sysmex XN 2000. It measures erythrocytes with low hemoglobin concentration. The aim of this study was to verify whether the research parameter HYPO-He should be included as a diagnostic analysis for anemia at the Hudiksvall Clinical Chemistry Laboratory in the Gävleborg Region, Sweden. The study material consisted of 34 samples, whole blood with EDTA. Two samples for in-series-imprecision and two for sustainability control. The two control materials (low and normal) and samples with properly filled tubes were analyzed with Sysmex XN in the reticulocyte channel using the optical method, by flow cytometry with laser. The results of duplicate sample on Sysmex XN showed that linear regression value R2 was 1.0 and coefficient of variation CV was 0.83 %. During this project, similar results were obtained that other projects had done with different instruments and methods. According to the results, HYPO-He is reliable and useful for improved iron deficiency diagnostics. Additional studies on HYPO-He stability is recommended