Alternative splicing (AS) modulates many physiological and pathological processes. For instance, AS of the BCL-X gene balances cell survival and apoptosis in development and cancer. Herein, we identified the polypyrimidine tract binding protein (PTBP1) as a direct regulator of BCL-X AS. Overexpression of PTBP1 promotes selection of the distal 5' splice site in BCL-X exon 2, generating the pro-apoptotic BCL-Xs splice variant. Conversely, depletion of PTBP1 enhanced splicing of the anti-apoptotic BCL-XL variant. In vivo cross-linking experiments and site-directed mutagenesis restricted the PTBP1 binding site to a polypyrimidine tract located between the two alternative 5' splice sites. Binding of PTBP1 to this site was required for its effect...
Polypyrimidine tract binding protein (PTBP1) is a widely expressed RNA binding protein that acts as ...
The Bcl-2 family of proteins are regulators of apoptosis. One of the Bcl-2 genes, Mcl-1, produces tw...
Alternative splicing (AS) is a robust generator of mammalian transcriptome complexity. Splice site s...
Alternative splicing (AS) modulates many physiological and pathological processes. For instance, AS ...
<div><p>Alternative splicing (AS) provides a potent mechanism for increasing protein diversity and m...
Alternative splicing (AS) provides a potent mechanism for increasing protein diversity and modulatin...
Alternative splicing (AS) provides a potent mechanism for increasing protein diversity and modulatin...
Most human genes generate multiple protein isoforms through alternative pre-mRNA splicing, but the m...
Many RNA-binding proteins including a master regulator of splicing in developing brain and muscle, p...
Splicing is an essential step in gene regulation to produce mature mRNA from a messenger RNA precurs...
Alternative pre-mRNA splicing (AS) provides an efficient means for diversifying the proteome and reg...
Alternative pre-mRNA splicing (AS) plays a major role in gene expression in higher eukaryotes, espec...
Polypyrimidine tract binding protein (PTB) is known to silence the splicing of many alternative exon...
The Bcl-2 family of proteins are the primary regulators of the intrinsic apoptotic pathway, with ant...
Alternative splicing is an important form of gene regulation during tissue development. In the mamma...
Polypyrimidine tract binding protein (PTBP1) is a widely expressed RNA binding protein that acts as ...
The Bcl-2 family of proteins are regulators of apoptosis. One of the Bcl-2 genes, Mcl-1, produces tw...
Alternative splicing (AS) is a robust generator of mammalian transcriptome complexity. Splice site s...
Alternative splicing (AS) modulates many physiological and pathological processes. For instance, AS ...
<div><p>Alternative splicing (AS) provides a potent mechanism for increasing protein diversity and m...
Alternative splicing (AS) provides a potent mechanism for increasing protein diversity and modulatin...
Alternative splicing (AS) provides a potent mechanism for increasing protein diversity and modulatin...
Most human genes generate multiple protein isoforms through alternative pre-mRNA splicing, but the m...
Many RNA-binding proteins including a master regulator of splicing in developing brain and muscle, p...
Splicing is an essential step in gene regulation to produce mature mRNA from a messenger RNA precurs...
Alternative pre-mRNA splicing (AS) provides an efficient means for diversifying the proteome and reg...
Alternative pre-mRNA splicing (AS) plays a major role in gene expression in higher eukaryotes, espec...
Polypyrimidine tract binding protein (PTB) is known to silence the splicing of many alternative exon...
The Bcl-2 family of proteins are the primary regulators of the intrinsic apoptotic pathway, with ant...
Alternative splicing is an important form of gene regulation during tissue development. In the mamma...
Polypyrimidine tract binding protein (PTBP1) is a widely expressed RNA binding protein that acts as ...
The Bcl-2 family of proteins are regulators of apoptosis. One of the Bcl-2 genes, Mcl-1, produces tw...
Alternative splicing (AS) is a robust generator of mammalian transcriptome complexity. Splice site s...