We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for future point-of-care diagnosis of malaria. In the developed setup, specific detection of malaria parasites in crude blood samples is facilitated by the conversion of single Plasmodium falciparum topoisomerase I (pfTopI) mediated cleavage-ligation events, happening within nanometer dimensions, to micrometer-sized products readily detectable at the single molecule level in a fluorescence microscope. In principle, REEAD requires no special equipment and the readout is adaptable to simple colorimetric detection systems. Moreover, with regard to detection limit the presented setup is likely to outcompete standard gold immuno-based diagnostics. Hen...
Malaria remains one of the most prevalent infectious diseases and results in significant mortality. ...
Background: Isothermal amplification techniques are emerging as a promising method for malaria diagn...
BACKGROUND: To detect all malaria infections in elimination settings sensitive, high throughput and ...
We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for ...
The so-called Rolling Circle Amplification allows for amplification of circular DNA structures in a ...
Rapid and reliable diagnosis is essential in the fight against malaria, which remains one of the mos...
We present an attractive new system for the specific and sensitive detection of the malaria-causing ...
We present an attractive new system for the specific and sensitive detection of the malaria-causing ...
We present an attractive new system for the specific and sensitive detection of the malaria-causing ...
Abstract Background Five species of Plasmodium are known to infect humans. For proper treatment of m...
A high-sensitivity assay has been developed for the detection of human topoisomerase I with single m...
Malaria diagnosis methods offering species-specific information on the causative parasites, along wi...
The study of interaction between human Topoisomerase I (hTopo I) and the appropriate sequences of DN...
Highly sensitive and field deployable molecular diagnostic tools are critically needed for detecting...
Loop-mediated isothermal amplification (LAMP) of DNA offers the ability to detect very small quantit...
Malaria remains one of the most prevalent infectious diseases and results in significant mortality. ...
Background: Isothermal amplification techniques are emerging as a promising method for malaria diagn...
BACKGROUND: To detect all malaria infections in elimination settings sensitive, high throughput and ...
We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for ...
The so-called Rolling Circle Amplification allows for amplification of circular DNA structures in a ...
Rapid and reliable diagnosis is essential in the fight against malaria, which remains one of the mos...
We present an attractive new system for the specific and sensitive detection of the malaria-causing ...
We present an attractive new system for the specific and sensitive detection of the malaria-causing ...
We present an attractive new system for the specific and sensitive detection of the malaria-causing ...
Abstract Background Five species of Plasmodium are known to infect humans. For proper treatment of m...
A high-sensitivity assay has been developed for the detection of human topoisomerase I with single m...
Malaria diagnosis methods offering species-specific information on the causative parasites, along wi...
The study of interaction between human Topoisomerase I (hTopo I) and the appropriate sequences of DN...
Highly sensitive and field deployable molecular diagnostic tools are critically needed for detecting...
Loop-mediated isothermal amplification (LAMP) of DNA offers the ability to detect very small quantit...
Malaria remains one of the most prevalent infectious diseases and results in significant mortality. ...
Background: Isothermal amplification techniques are emerging as a promising method for malaria diagn...
BACKGROUND: To detect all malaria infections in elimination settings sensitive, high throughput and ...