The erythroblastic island (EBI) is a multicellular structure forming an erythropoietic niche consisting of a central macrophage surrounded by a rosette of maturing erythroblasts. Since their discovery more than 60 years ago, simultaneous quantification and visualization of EBIs remain difficult. Although flow cytometry enables high-throughput quantification of cell aggregates co-expressing macrophage and erythroblast markers, it cannot visually confirm whether the aggregates are genuine EBIs. While immunofluorescence microscopy allows visualization of EBIs, its low throughput limits its use for quantification. In the current study we employed nine-channel imaging flow cytometry (IFC) to develop a method to directly visualize and quantify EB...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
<p>Colonies were scored by inverted fluorescent microscopy and faint background fluorescence of nega...
Globally, an estimated 25% of the global population suffer from anaemia. Despite extensive research ...
Background: Accumulating data support the role of flow cytometry (FCM) in diagnostic work-up of myel...
<p>(<b>A</b>, <b>B</b>) Representative flow cytometric profiles of erythroid-lineage cells in the BM...
Haematopoiesis is the process of blood production arising from haematopoietic stem cells (HSCs) that...
<p>(A) Representative contour plots from flow cytometric analyses of myeloid (CD11b<sup>+</sup>), B ...
The cytokines granulocyte colony-stimulating factor (G-CSF) and Flt3 ligand (Flt3-L) mobilize hemato...
Bone marrow contains numerous different cell types arising from hematopoietic stem cells (HSCs) and ...
Similarly to other tissues, the bone marrow contains subsets of resident tissue macrophages, which a...
<p>Each point on the scatter plots represents data from a single mouse (o = <i>Drf1</i><sup>−/−</sup...
Resident bone marrow macrophages provide a microenvironment for erythropoiesis, forming erythroblast...
<p>(A) Single spleen cells suspensions from CBA/Pk and Char10C mice were labelled with antibodies ag...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
<p>Colonies were scored by inverted fluorescent microscopy and faint background fluorescence of nega...
Globally, an estimated 25% of the global population suffer from anaemia. Despite extensive research ...
Background: Accumulating data support the role of flow cytometry (FCM) in diagnostic work-up of myel...
<p>(<b>A</b>, <b>B</b>) Representative flow cytometric profiles of erythroid-lineage cells in the BM...
Haematopoiesis is the process of blood production arising from haematopoietic stem cells (HSCs) that...
<p>(A) Representative contour plots from flow cytometric analyses of myeloid (CD11b<sup>+</sup>), B ...
The cytokines granulocyte colony-stimulating factor (G-CSF) and Flt3 ligand (Flt3-L) mobilize hemato...
Bone marrow contains numerous different cell types arising from hematopoietic stem cells (HSCs) and ...
Similarly to other tissues, the bone marrow contains subsets of resident tissue macrophages, which a...
<p>Each point on the scatter plots represents data from a single mouse (o = <i>Drf1</i><sup>−/−</sup...
Resident bone marrow macrophages provide a microenvironment for erythropoiesis, forming erythroblast...
<p>(A) Single spleen cells suspensions from CBA/Pk and Char10C mice were labelled with antibodies ag...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
Figure S1. Gating used to identify endothelial cells (ECs), mesenchymal cells (MSCs), osteoblasts (O...
<p>Colonies were scored by inverted fluorescent microscopy and faint background fluorescence of nega...