A sequential algorithm for the blind separation of a class of periodic source signals is introduced in this paper. The algorithm is based only on second-order statistical information and exploits the assumption that the source signals have distinct periods. Separation is performed by sequentially converging to a solution which in effect diagonalizes the output covariance matrix constructed at a lag corresponding to the fundamental period of the source we select, the one with the smallest period. Simulation results for synthetic signals and real electrocardiogram recordings show that the proposed algorithm has the ability to restore statistical independence, and its performance is comparable to that of the equivariant adaptive source separat...