Temperature as a parameter in HPLC optimisation

This study focused on the capabilities of elevated temperature chromatography and its potential applications. The effects of separation temperature on compound retention, selectivity and efficiency were studied. The change in retention with temperature was explored using a number of test compound solutions. A detailed investigation of the importance of system variables like dead volume, mobile phase composition, different stationary phases, and temperature control of column and eluent was performed. The effects of different eluents and different means of temperature control on the separation selectivity and efficiency were investigated and results were compared. [Continues.