Biogenic amine formations in wet and spray-dried fish (Equulites klunzingeri) silage prepared with lactic acid bacteria strains

Fish silage is an effective method to produce high quality animal food from discard fish. Lactic acid bacteria utilization for the production of fish silages have considerable advantages than the chemical silages because of their positive properties such as organic acids, bacteriocins productions and the enhancement of the flavour. However, fish products may contain biogenic amines because of the action of endogenous and bacterial decarboxylase enzymes activities. lt was reported that some lactic acid bacteria (LAB) were able to degrade biogenic amines by means of amino oxidases. ln this study, the effects of lactic acid bacteria on biogenic amines in wet and spray-dried fish silages for the duration of the fermentation process were investigated. The fish (Equulites klunzingeri) silages were prepared with LAB strains (Lactobacillus plantarum, Lactobacillus brevis, Pediococcus acidilactici, Enterococcus gallinarum and Streptococcus spp.) and formic acid as the control group. Acid and fermented silage groups were stored at room temperature (27-28oC\ for three weeks. After three weeks, all silage groups were spray-drieri L;siiig mini spray dryer (Buchi-290,Switzerland). Biogenic amine analysis in wet and dried fish silages was performed using a rapid HPLC method. This project was supported by Scientific and Technological Resea rch Council of Turkey (TOVAG-2 13O166)