Evaluation and Comparison of A New Type of Temporary Immersion System (TIS) Bioreactors for Myrtle (Myrtus communis L.)

Myrtle is known to be an important aromatic and medicinal plant species. Myrtle plant isconsumed mainly as spice having several uses in the perfumery, food and pharmaceutical industries. It isalso widely used for ornamental purposes such as fencing, roadsides, and green cutting. Plant tissueculture techniques are an essential tool to micropropagate for the plant producer. TIS (Temporaryimmersion systems) is an alternative plant tissue culture technique for micropropagation mechanism inplant species. A new culture vessel named PlantForm TIS bioreactor has been recently developed. In thepresent study, we aimed to evaluate and compare a new type of TIS bioreactors with agar media formyrtle. MS (Murashige and Skoog) medium supplemented with 1 mg l-1 BAP (benzylaminopurine) wasused for multiplication, with 1 mg l-1IBA (indole-3-butyric acid) for rooting. Two different immersiontimes (4 h and 8 h) were compared with the solid agar medium. Plantlets growing in PlantForm systemsperformed better than those in the solid medium. All data recorded at micropropagation and rootingtreatments were much more successful. In conclusion, PlantForm TIS bioreactor presents a validalternative to traditional in vitro micropropagation and rooting systems, resulting in a reduction of cost,labor, and time for the mass propagation