Petroleum Ether and Chloroform Soluble Fractions of Whole Plant Extract of Acanthus ilicifolis Linn. Possesses Potential Analgesic and Antioxidant Activities.

Background: Medicinal plants are the major sources of traditional treatment of disease and new drug discovery due to major side effects of synthetic drug. Objectives: The aim of study was to evaluate analgesic and antioxidant activities of petroleum ether and chloroform soluble fractions of whole plant extract of Acanthus ilicifoius. Materials and Methods: The plant extract, standard diclofenac, and distilled water as control was administered post orally in Swiss albino mice and observe the analgesic activity by acetic acid (0.6%) induced writhing method. The plant extract was also subjected to perform reducing power assay, DPPH free radical scavenging activity, and FRAP assay to evaluate antioxidant activity. Results: The pet ether, and chloroform soluble fraction of plant extract revealed significant analgesic activity on mice model. Notably, the pet ether and chloroform fraction showed (40.14 ± 2.32) % and (40.12 ± 0.9) % analgesic inhibition, whereas standard diclofenac revealed (52.79 ± 2.62) % analgesic inhibition. In antioxidant activity assay, the plant extract showed mild to moderate antioxidant activities compare to standard ascorbic acid. Conclusion: From the results, it could be concluded that, the pet ether and chloroform fractions of whole plant extract of A. ilicifolius possesses potential analgesic and antioxidant properties