Investigation of inhibition effects of some natural phenolic compounds on Glutathione S-transferase (GST), Acetylcholinesterase (AChE), Butyrylcholinesterase (BChE), ?-amylase, and ?-glycosidase: Antidiabetic, anticholinergics, antiparasitic study

Summary: Herein, we report the experimental results of the inhibition effects of some phenolic compounds such as 2,4,6-Trihydroxybenzaldehyde, 3,4-Dihydroxy-5-methoxybenzoic acid, Serotonin hydrochloride on ?-amylase, ?-glycosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and glutathione S-transferase (GST) enzymes. ?-Amylase from porcine pancreas, ?-glycosidase from Saccharomyces cerevisiae, glutathione s-transferase from human placenta, acetylcholinesterase from human erythrocytes and Butyrylcholinesterase from equine serum were used as enzymes. These enzymes play a very important role in metabolism. In this study the Ki values obtained by the experiments done for the compounds on some enzymes such as GST, AChE, BChE, ?-amylase and ?-glycosidase were found in the range of 0.31±0.5-1.02±0.3 µM, 484.03±45.45-802.83±104.3 µM, 238.22±67.31-482.73±125.6 µM and 9.28±0.56-11.23±3.43 µM, respectively. The experiment results showed that the ?-amylase enzyme has a selective inhibitory effect and its IC50 values were found to be in the range of 0.92-9.63 µM. As a result, it was found that these phenolic compounds have a selective effect on ?-amylase, ?-glycosidase, AChE, BChE and GST enzymes, and they can be used as antiparasitic, antidiabetic and anticholinergic. © 2019 Chemical Society of Pakistan. All rights reserved