Production of Cephalosporin C from Acremonium chrysogenum, and its Antimicrobial Activity against Some Pathogenic Bacteria

The Production of cephalosporin C by Acremoniumchrysogenumin flask contain fermentation medium, after that its extraction, purification and estimation was performedby Spectrophotometric Method.The antimicrobial activities of both the crude and purified cephalosporin C were performed against the test organisms by Agar Well Diffusion Method.Agar well diffusion method and the inhibition zones were recorded and evaluated the effectiveness of the antibiotic against gram positive bacteria (Staphylococcus aereusand Bacillus subtilis) and no effectiveness against gram negative bacteria (Pseudomonas aeruginosaand Proteus mirabilis).The Production of cephalosporin C by Acremoniumchrysogenumin flask contain fermentation medium, after that its extraction, purification and estimation was performedby Spectrophotometric Method.The antimicrobial activities of both the crude and purified cephalosporin C were performed against the test organisms by Agar Well Diffusion Method.Agar well diffusion method and the inhibition zones were recorded and evaluated the effectiveness of the antibiotic against gram positive bacteria (Staphylococcus aereusand Bacillus subtilis) and no effectiveness against gram negative bacteria (Pseudomonas aeruginosaand Proteus mirabilis)