Notch1 is asymmetrically distributed from the beginning of embryogenesis and controls the ventral center
- Castro Colabianchi, Aitana Manuela
- Revinski, Diego Raul
- Encinas, Paula Inés
- Baez, Maria Veronica
- Monti, Renato José
- Rodríguez Abinal, Mateo
- Kodjabachian, Laurent
- Franchini, Lucia Florencia
- Lopez, Silvia Liliana
DOIAbstract
Based on functional evidence, we have previously demonstrated that early ventral Notch1 activity restricts dorsoanterior development in Xenopus. We found that Notch1 has ventralizing properties and abolishes the dorsalizing activity of β-catenin by reducing its steady state levels, in a process that does not require β-catenin phosphorylation by glycogen synthase kinase 3β. In the present work, we demonstrate that Notch1 mRNA and protein are enriched in the ventral region from the beginning of embryogenesis in Xenopus. This is the earliest sign of ventral development, preceding the localized expression of wnt8a, bmp4 and Ventx genes in the ventral center and the dorsal accumulation of nuclear β-catenin. Knockdown experiments indicate that No...
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