Process-based Modelling of RNA and Protein Interactions: A Formal Approach

Our work is focussed on the computational study of the molecular interactions in biological systems. We hypothesise the use of process algebras to highlight the relation between the complexity of the functions carried out by a biological entity and the type of interactions tying the elementary units that compose its structure. This approach is intended to define predictive models able to generate new knowledge, on the system itself, complementary to the one obtained via empirical methods. We investigated the way in which the interactions between nucleotides determine the three-dimensional conformation of RNAs and hence their functions. With the aid of formal models based on process algebras, we compared the folding process of proteins with the one performed by RNAs. We formally proved the existence of an abstraction level in which these two kinds of processes show a congruence in their behaviour. Such result allows us to identify and model the distinguishing features of the studied biological processes only on the basis of the known properties of the interactions that bind the nucleotides (in RNAs) and the amino acids (in proteins). This was possible thanks to the expressiveness of a specific process algebra, the Milner’s CCS (Calculus of Communicating Systems)1. The formal demonstration of the existence of the above-described abstraction level has been provided in an article submitted for publication in a peer-reviewed journal2. We are also working on an application of the proposed modelling approach to the studies carried out at the CPT (Centre de Physique Théorique, Aix-Marseille University) on the long-distance electrodynamic interactions of biomolecules3. The main idea is to develop a simulator able to solve the problem of the possible interpenetrations of the represented molecules by providing them with three-dimensional shapes that cannot cross each other. It would be also intended to yield information on the temporal evolution of the molecular interactions. References: 1) Milner, R. Communication and Concurrency; Upper Saddle River, 1989, NJ, USA: Prentice-Hall, Inc. 2) Maestri, S.; Merelli, E. Process calculi may reveal the equivalence lying at the heart of RNA and proteins. 2018, Manuscript submitted for publication. 3) Gori, M. et al. Investigation of Brownian diffusion and long-distance electrodynamic interactions of biomolecules. Noise and Fluctuations (ICNF), 2015, International Conference on. IEEE, pp. 1 – 4