Assessment of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Pathogenic Bacteria from Respiratory Samples in Patients with Hospital-Acquired Pneumonia
- Vergara, Andrea
- Boutal, Hervé
- Ceccato, Adrian
- López, Miriam
- Cruells, Adrián
- Bueno, Leticia
- Moreno-Morales, Javier
- Puig de la Bellacasa, Jordi
- Castro, Pedro
- Torres Martí, Antoni
- Marco Reverté, Francesc
- Casals Pascual, Climent
- Vila Estapé, Jordi
DOIAbstract
Rapid identification of the causative agent of hospital-acquired pneumonia (HAP) will allowan earlier administration of a more appropriate antibiotic and could improve the outcome of thesepatients. The aim of this study was to develop a rapid protocol to identify the main microorganismsinvolved in HAP by loop-mediated isothermal amplification (LAMP) directly from respiratory samples.First of all, a rapid procedure (<30 min) to extract the DNA from bronchoalveolar lavage (BAL),endotracheal aspirate (EA) or bronchoaspirate (BAS) was set up. A specific LAMP forStaphylococcusaureus,Escherichia coli,Klebsiella pneumoniae, Pseudomonas aeruginosa,Stenotrophomonas maltophiliaandAcinetobacter baumanniiwas performed with the extracted solution at 65◦...
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