A Method for Selective Depletion of Zn(II) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(II) Deficiency

We describe the preparation, evaluation, and application of an S100A12 protein-conjugated solid support, hereafter the "A12-resin", that can remove 99% of Zn(II) from complex biological solutions without significantly perturbing the concentrations of other metal ions. The A12-resin can be applied to selectively deplete Zn(II) from diverse tissue culture media and from other biological fluids, including human serum. To further demonstrate the utility of this approach, we investigated metabolic, transcriptomic, and metallomic responses of HEK293 cells cultured in medium depleted of Zn(II) using S100A12. The resulting data provide insight into how cells respond to acute Zn(II) deficiency. We expect that the A12-resin will facilitate interrogation of disrupted Zn(II) homeostasis in biological settings, uncovering novel roles for Zn(II) in biology. Keywords: peptides and proteins; metals; organic polymers; cell cultureIonsNational Institute of Environmental Health Sciences (P30-ES002109)National Institutes of Health (U.S.) (Grant R01- AR071443)National Institutes of Health (U.S.) (Grant R01-GM065519)National Science Foundation (U.S.) (CHE-1352132)National Institutes of Health (U.S.) (T32- EB019940)National Cancer Institute (U.S.) (Grant P30- CA14051