An effective new route for synthesizing branched oligoribonucleotides in the solid phase in the 5′ to 3′ direction has been developed. This required the synthesis of reversed monomers, viz. protected nucleoside 5′-phosphoramidites bearing 2′-O-Fpmp and 3′-O-pixyl protecting groups as well as special branch-point monomers, viz. protected nucleoside 5′-phosphoramidites bearing either 2′,3′-O-dipixyl protection in the case of adenosine, cytidine and uridine, or 2′,3′-O-dilaevulinyl protection in the case of guanosine. These monomers are assembled on commercial synthesizers into branched oligoribonucleotides in high yield, the crude products are readily purified by reversed-phase HPLC whilst still partially protected, and the fully deprotected ...
The first part of the work describes a procedure of oligonucleotide purification using a reversed-ph...
International audienceBranched oligonucleotides containing a biologically relevant DNA lesion, dCyd3...
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonuc...
An effective new route for synthesizing branched oligoribonucleotides in the solid phase in the 5′ t...
An effective new route for synthesizing branched oligoribonucleotides in the solid phase in the 5′ t...
The phenoxyacetyl group was investigated as a base protecting group in oligoribonucleotide synthesis...
The generation of synthetic oligonucleotides is dependent on an efficient solid-phase synthesis meth...
In studies aimed at improving the automated solid-phase synthesis of RNA, a new protecting group, 4-...
The recent discovery that small interfering RNAs (siRNAs) induce gene suppression in mammalian cells...
Two strategies for the construction of chemically modified, DNA cleaving oligoribonucleotide probes ...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The chemical synthesis of RNA requires an efficient protecting system for the 2$\sp\prime$-hydroxyl ...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The first part of the work describes a procedure of oligonucleotide purification using a reversed-ph...
International audienceBranched oligonucleotides containing a biologically relevant DNA lesion, dCyd3...
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonuc...
An effective new route for synthesizing branched oligoribonucleotides in the solid phase in the 5′ t...
An effective new route for synthesizing branched oligoribonucleotides in the solid phase in the 5′ t...
The phenoxyacetyl group was investigated as a base protecting group in oligoribonucleotide synthesis...
The generation of synthetic oligonucleotides is dependent on an efficient solid-phase synthesis meth...
In studies aimed at improving the automated solid-phase synthesis of RNA, a new protecting group, 4-...
The recent discovery that small interfering RNAs (siRNAs) induce gene suppression in mammalian cells...
Two strategies for the construction of chemically modified, DNA cleaving oligoribonucleotide probes ...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The chemical synthesis of RNA requires an efficient protecting system for the 2$\sp\prime$-hydroxyl ...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The synthesis of base protected 5′-O-dimethoxytrityl-2′-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-y...
The first part of the work describes a procedure of oligonucleotide purification using a reversed-ph...
International audienceBranched oligonucleotides containing a biologically relevant DNA lesion, dCyd3...
The [(triisopropylsilyl)oxy]methyl (TOM) group is a useful protecting group for the 2′‐OH of ribonuc...