The Expression of IL-1β gene In Response to Mannheimia haemolytica Bacteria in Sheep Alveolar Macrophages in vitro

Small ruminants, particularly sheep, are suffering from many respiratory diseases.Among the causative agents of respiratory diseases, the most important microorganismsare Mannheimia haemolytica. In the case of a most severe form of infection which iscommonly known as the severe fibrinous pleuropneumonia characterized by fibrindeposition and merging, intra-alveolar hemorrhage, forceful leukocyte infiltration inalveoli of the lungs. Being a Gram-negative bacterium, Mannheimia haemolytica iscausing respiratory diseases in animals and pneumonia which is the must dangersdiseases that make a great economic loss. The sheep immune system is responsible forthe detection, capturing and elimination of foreign bodies including bacteria. However,there are no substantial studies explaining the molecular mechanism of respiratorydiseases and immune system response in sheep lung. Therefore, this study wasconducted to investigate the expression pattern of IL-1β, one of the important genes inimmune system, to understand the molecular mechanism underlying in M. haemolyticainduced infection in sheep lung.