Influence of optical clearing on collagen crosslinking of sclera

The dynamics of the change of dimensions (cross-section area) of porcine sclera specimens in the process of optical clearing was measured using optical coherence tomography (OCT). Based on the obtained data, the optimal (corresponding to maximum tissue contraction) time interval between the application of the immersion agent and UV/riboflavin photo-crosslinking was found. The ability of multiphoton microscopy (tomography) to detect morphological changes in scleral tissue subjected to UV/riboflavin crosslinking in optically cleared state (with subsequent rehydration) was discussed based on measured BSHG images of UV/riboflavin treated porcine sclera ex vivo