Carvedilol, Tablets Assay Method Development and Validation by UV Spectroscopy, RP-HPLC and HPTLC

Aim & Objectives : The present study describes the development of a new rapid, simple, sensitive and reproducible RP-HPLC, HPTLC, UV Spectrophotometry method for the analysis of Carvedilol that offer certain advantages in its simplicity and sensitivity and applicable in routine analysis. Material & Methods: Carvedilol is polar in nature, reverse phase chromatography has been used and assay method is developed for UV-Spectroscopy, RP-HPLC, & HPTLC & method validation is also done. Results & Discussion: The validated method was applied for the analysis of tablet containing 25 mg carvedilol drug as the label claim. The method developed was simple. It has showed a good peak and good Rf values Conclusion: In order to develop a RP-HPLC, HPTLC, UV effective most of the effect should be spent in method development and optimization as this will improve the final method performance. A well-developed method should be easy to validate. A method should be developed with the goal to analyze rapidly, the preclinical samples, formulations and commercial samples.