Proton induced fluorescence microscopy of single living cells utilizing advanced microbeam irradiation system (SPICE)

Advanced microbeam irradiation system made it possible to deliver a defined amount of radiation on a single cell with a few micrometer resolution. Our microbeam, the Single-Particle Irradiation system to CEll (SPICE) provides a 3.4 MeV proton microbeam focused with a quadrupole magnetic lens on an upward vertical beam line (1, 2). SPICE is the only proton microbeam in Japan that can target nucleus, cytoplasm, or both with high throughput, high targeting accuracy along with promising stability. In this presentation, an accurate and systematic investigation of the SPICE microbeam profile using novel fluorescent nuclear track detectors (FNTDs, Al2O3: C, Mg) will be presented along with our recent results from single cell analysis of microbeam targeted cells on defensive signalling through intra- and inter-cellular response against radiation, such as cytoplasmic damage response (3, 4), and cell-to-cell communication between human normal and carcinoma cells (5).1. T. Konishi, et al., J Radiat Res 54(2013) 736-747.2. D. Ohsawa, et al., Nucl Instrum Methods Phys. Res B 453 (2019) 9-14.3. J. Wang and T. Konishi, Cancer Sci, 110 (2019) 686-696.4. T. Konishi, et al., J. Radiat Cancer Res 9 (2018)183-189.5. A. Kobayashi, et al., Radiat Prot Dosim 183 (2019) 142-146.ABiS Symposium Forefront and Future of Electron Microscopic Imaging 電子顕微鏡イメージングの最先端と未