Role of natural surfactants in bioremediation and bioavailability of PAH

Motivation: Polycyclic aromatic hydrocarbons (PAHs) are organic compounds found in most polluted areas mainly due to anthropogenicactivity. Such compounds entail several damages to human health and the environment in general because of their toxicityand carcinogenecity. Moreover, due to their recalcitrance and ubiquity, they supose a problem where research groups focustheir attention(1). In our case, efforts to find solutions to PAH pollution are aimed at bioremediation, looking for chemicalstrategies for improving the bioavailability in the biorremediation of contaminated areas, always without increasing the risk tothe environment where they are adsorbed.The main objectives of this project are the tuning of the fluorimetry method to detect hydrocarbons in aqueous samples and totest the effectiveness of a novel commercial rhamnolipid versus another one previously used, obtained from a cell culture atthe laboratory.Methods: We used a strain of Mycobacterium gilvum as biodegrader organism, and we have tested its ability to use these compoundsas carbon source (1, 2) in biodegradation experiments against different concentrations of pyrene (4-rings PAH as arepresentative compound of this group) supplied dissolved and crystalline in aqueous solutions. The variation in theconcentration of pyrene is measured using fluorimetry techniques in an equipment able to submit a sample to ultravioletradiation and collect the radiation emitted by the sample in a graph, where we will be able to detect a peak representing thepyrene in a specific and known length wave. We also studied the effect of a commercial rhamnolipid recently bought by thelaboratory, which is expected to increase the bioavailability of such compounds due to its surfactant effect, thus overcomingone of the main barriers to remediation and being a friendly alternative with the environment because of its naturally origin (2).Results: The bacteria is able to assimilate the supplied pyrene. When rhamnolipids are added, the final concentration of pyreneremaining in the saturated samples is less than in the cases we are not providing the rhamnolipid. When the pyrene issupplied as crystals, passive dosing occurs so that it dissolves until equilibrium is reached. When M. gilvum is added in thatpoint, pyrene is removed from the aqueous phase by breaking the balance and allowing a greater amount of crystals to bedissolved and removed from the medium.Conclusions: It has been found that using this commercial rhamnolipid is more effective, because it allows a greater solubility and theadvantage of having it available simply and quickly