Effect of nerolidol on the level of intracellular calcium in bladder carcinoma cell line (TCCSUP) : bachelor thesis

Kalcij je najobilniji mineral u ljudskom tijelu. 99% kalcija u tijelu se nalazi u kostima i zubima dok se ostalih 1% nalazi u krvi i mekom tkivu. Tih 1% ima važnu ulogu u biološkim procesima i njegova koncentracija u stanicama je strogo regulirana paratireoidnim hormonom i kalcitriolom. U stanici se nalazi u većim koncentracijama u mitohondrijima i endoplazmatskom retikulumu koji služi kao unutarstaniĉno skladište za tijesnu regulaciju njegove koncentracije. Izvanstaniĉni kalcij služi kao regulator razlike potencijala u staniĉnim membranama. Nerolidol je seskviterpenski alkohol prisutan u mnogim eteriĉnim uljima razliĉitih biljaka cvjetnog mirisa. Zbog njegove prisutnosti u ĉovjekovoj svakodnevnici znanstvenici su se zainteresirali za ispitivanje njegovih bioloških svojstava. Razni utjecaji nerolidola na tumorske stanice već su ispitani i dali su zanimljive rezultate potiĉući daljna istraživanja. Zanimljivo je ispitati utjecaj nerolidola na koncentraciju unutarstaniĉnog kalcija kod raznih vrsta tumorskih stanica. Poremećaji u regulaciji kalcijem u stanici dovode do slijeda staniĉnih odgovora koji vode apoptozi, to jest induciranoj programiranoj staniĉnoj smrti stanica što svakako jest cilj antitumorske terapije. Pozitivnim odgovorom stanica koliĉinom unutarstaniĉnog kalcija u ovom eksperimentu otvaraju se vrata daljnjim istraživanjima utjecaja nerolidola na zdrave i tumorske stanice. Podaci su prikupljeni snimanjem fluorescencije kelata kalcija i fluorescentnog reagensa Fluo-3 u stanicama karcinoma mokraćnog mjehura (TCCSUP) tijekom tretiranja razliĉitim koncentracijama nerolidola.Calcium is the most abundant mineral in human body. 99% of calcium in body is found in bones and teeth while the rest 1% is found in blood and soft tissue. That 1% plays an important role in biological processes and it's concentration is strongly regulated in cells with parathyroid hormone and calcitriol. In cells, it is found in greater concentrations in mitochondrium and endoplasmatic reticulum which serves as intracellular storage for its regulation. Extracellular calcium serves as a regulator of potential difference in cell membranes. Nerolidol is sesquiterpene alcohol naturaly occuring in many esential oils of different plants with floral odor. Becouse of its presence in human everyday life, scientists became interested in examination of its biological properties. Different effects of nerolidol on tumorigenic cells have been examined and they gave interesting results encourageing further researches. It is interesting to examine the effect of nerolidol on the amount of intracellular calcium in different types of human cancer cells. Disorders in calcium regulation triggers cascade of cellular events that lead to apoptosis, induced programed cell death which is the goal of every anticancer therapy. With positive cell response in the change of the amount of intracellular calcium in this experiment it opens the doors to furher researches in nerolidol effects on healthy and tumorigenic cells. Datas were collected filming fluorescence of chelate of calcium and fluorescentic reagent Fluo-3 with flow cytometer Accuri C6 in human bladder carcinoma cells (TCCSUP) while being treated with different concentrations of nerolidol