Detection of gasoline from internal tissues for use in determining victim status at the time of a fire

In Ontario, fire investigators from the Office of the Fire Marshal (OFM) are responsible for determining the origin and cause of suspicious fires. As part of the investigation, fire debris samples are collected from the scene and analyzed by the Centre of Forensic Sciences. The standard practice is to collect items that are porous, highly absorbent or adsorbent with high surface areas as they allow for better retention of the ignitable liquids. The evidence typically collected includes carpets, cardboards, soils, cloths and other items that have not been impinged by flame such as beneath baseboards. These samples are analyzed for the presence of ignitable liquid residues which may be evidence that an accelerant was used at the fire. When a body is recovered from a fire it can provide another source from which to collect samples for analysis. These samples can be especially helpful in instances where the fire generated an intense heat which may cause a loss of ignitable liquid residues from the fire debris. The tissue samples have a greater likelihood of still containing residues as the organs and body fluids can act as a shield protecting the residues from volatilization. The purpose of this study is to validate whether a victim was alive or deceased at the time a fire was intentionally set by detecting presence or absence of gasoline residues within their lungs and heart blood post fire. It was hypothesized that only when a victim was alive and performing respiration would sufficient gasoline vapours enter the airways and bloodstream for detection postmortem. Contamination becomes a significant issue when these samples are collected at autopsy and this study aimed to determine the accuracy with which a gasoline signature can be interpreted following the collection and analysis of lung tissue and heart blood. Pig (Sus domesticus) carcasses were chosen as acceptable analogues for humans in this study. The experiments involved anaesthetizing a pig (with Animal Ethics Approval), exposing the pig to gasoline vapours for 10 minutes, and then euthanizing it. The carcass was clothed with a cotton t-shirt and placed in a house where additional gasoline was poured onto it. The house also contained two additional clothed pig carcasses which did not inhale gasoline vapours; one with gasoline poured directly onto it and the other with no gasoline exposure (negative control). Thermocouples were placed under each carcass and in the centre of each room at ceiling and floor level to record the temperature. The house was set ablaze and monitored by a volunteer fire service. After the fire had reached V flashover and was suppressed, the carcasses were collected and their lungs and heart blood excised at a necropsy. The lungs and heart blood were then placed into glass mason jars following the OFM protocol. The headspace from each sample was analyzed by thermal desorption-gas chromatography-mass spectroscopy to determine the presence or absence of a gasoline signature. Two full scale house fires were conducted in order to obtain three replicates. The results showed that only the lungs and heart blood from the pig that inhaled gasoline contained gasoline residues. This indicates that it is possible to determine a victim???s status at the time of the fire based on the detection of gasoline in the lungs and/or heart blood. It was also concluded that contamination of samples during an autopsy can be minimized by changing gloves before handling the internal tissues. The thermal data showed that the bodies act as an insulator and protects the underside as the temperatures under the carcasses did not exceed 30???C while the room reached over 900???C at the first full scale house fire. These results will impact the forensic community by demonstrating the importance of analyzing a deceased victim???s internal tissues for ignitable liquid residues post fire as they may provide evidence of an intentionally set fire as well as providing information about the victim???s status when a fire was started. These findings will have a direct impact to the OFM as additional evidence can be obtained by completing internal tissue analysis. This will intern impact the Centre of Forensic Science (CFS) as it confirms the importance of analyzing internal tissues in order to provide results to fire investigators. Finally these findings should be used to implement new protocols at the Coroner???s Office so contamination can be minimized during fire autopsies and accurate samples are collected and sent to the CFS for analysis