MicroRNAs (miRNAs) modulate diverse biological and pathological processes via post-transcriptional gene silencing. High-throughput small RNA sequencing (sRNA-seq) has been widely adopted to investigate the functions and regulatory mechanisms of miRNAs. However, accurate quantification of miRNAs has been limited owing to the severe ligation bias in conventional sRNA-seq methods. Here, we quantify miRNAs and their variants (known as isomiRs) by an improved sRNA-seq protocol, termed AQ-seq (accurate quantification by sequencing), that utilizes adapters with terminal degenerate sequences and a high concentration of polyethylene glycol (PEG), which minimize the ligation bias during library preparation. Measurement using AQ-seq allows us to corre...
International audienceThe study of small RNAs (sRNAs) by next-generation sequencing (NGS) is challen...
Background: MicroRNAs (miRNAs) have been recognized as one of the key regulatory non-coding RNAs tha...
We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We...
IsomiRs, sequence variants of maturemicroRNAs, are usually detected and quantified using high-throug...
Advances in high-throughput sequencing have enabled profiling of microRNAs (miRNAs), however, a cons...
MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression. A...
microRNA (miRNA) expression profiles based on the highly powerful Illumina sequencing technology rel...
Many microRNAs regulate gene expression via atypical mechanisms, which are difficult to discern usin...
Recent advances in sequencing technology have helped unveil the unexpected complexity and diversity ...
Background: Massive parallel sequencing of transcriptomes revealed the presence of miRNA variants na...
MicroRNAs (miRNAs) are non-coding small RNAs which play a critical role in the regulation of gene ex...
Abstract The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for ...
>Background: Massive parallel sequencing of transcriptomes, revealed the presence of many miRNAs and...
The ongoing ascent of sequencing technologies has enabled researchers to gain unprecedented insights...
MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression. They are characterized by ...
International audienceThe study of small RNAs (sRNAs) by next-generation sequencing (NGS) is challen...
Background: MicroRNAs (miRNAs) have been recognized as one of the key regulatory non-coding RNAs tha...
We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We...
IsomiRs, sequence variants of maturemicroRNAs, are usually detected and quantified using high-throug...
Advances in high-throughput sequencing have enabled profiling of microRNAs (miRNAs), however, a cons...
MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression. A...
microRNA (miRNA) expression profiles based on the highly powerful Illumina sequencing technology rel...
Many microRNAs regulate gene expression via atypical mechanisms, which are difficult to discern usin...
Recent advances in sequencing technology have helped unveil the unexpected complexity and diversity ...
Background: Massive parallel sequencing of transcriptomes revealed the presence of miRNA variants na...
MicroRNAs (miRNAs) are non-coding small RNAs which play a critical role in the regulation of gene ex...
Abstract The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for ...
>Background: Massive parallel sequencing of transcriptomes, revealed the presence of many miRNAs and...
The ongoing ascent of sequencing technologies has enabled researchers to gain unprecedented insights...
MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression. They are characterized by ...
International audienceThe study of small RNAs (sRNAs) by next-generation sequencing (NGS) is challen...
Background: MicroRNAs (miRNAs) have been recognized as one of the key regulatory non-coding RNAs tha...
We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. We...