Erythrocyte membrane ATPases of AS and SS subjects

Erythrocyte membranes of normal, AS, and SS subjects were prepared and the specific activities of membrane ATPases of each were determined. Also G6PD activities of the above mentioned red cells were determined. The Na+KK+/Mg2+ dependent ATPase was significantly decreased in AS by 7% and by 5% in SS subjects as compared with controls. The Ca2+/Mg2+ ATPase activity was by far the most affected. A 9% increase in AS and 11% increase in SS subjects as compared with normals were observed. The Mg2+ ATPase showed variable results, an increase in AS, and a decrease in SS from control values being noted. Of ATPases in controls only the Mg2+ ATPase showed a significant variation between males and females. G6PD activity and pump enzymes of controls and AS and SS subjects showed no significant correlation, P > 0.05