Factors affecting the removal of aflatoxin M-1 from food model by Lactobacillus and Bifidobacterium strains

WOS: 000259366400011PubMed ID: 18803117This paper describes the ability of six dairy strains of Lactobacillus and Bifidobacterium to remove aflatoxin M-1 (AFM(1)) from phosphate-buffered saline (PBS) and reconstituted milk. Bacteria were incubated in both PBS and reconstituted milk containing 5, 10 and 20 ng mL(-1) for 0, 4 and 24 h at 37 degrees C. After centrifugation the concentration of AFM(1) was determined in the supernatant fraction using high-performance liquid chromatography. The binding abilities of AFM(1) by viable (10(8) CFU mL(-1)) and heat-killed Lactobacillus and Bifidobacterium strains in PBS ranged from 10.22 to 26.65% and 14.04 to 28.97%, respectively. Similarly, AFM(1)-binding capacity in reconstituted milk was found to range from 7.85 to 25.94% and from 12.85 to 27.31% for viable and heat-killed bacteria, respectively within 4 h. While B. bifidum Bb 13 was the best binder, the poorest removal was achieved by L. acidophilus NCC 68. Binding was reversible, and a small proportion of AFM(1) was released back into the solution. The toxin concentration and incubation period had no effect on the removal of AFM(1) by bacteria both in PBS and reconstituted milk.Cukurova University Research CouncilCukurova University [ZF2004D13]This study was supported by the Cukurova University Research Council (project no: ZF2004D13). We greatly thank Prof. Hans P. van Egmond from National Institute for Public Health and the Environment for supplying the AFM1 standard. We also thank A. Bozdogan for performing statistical analysis