Lamin protein gene expression in childhood acute myeloid leukemias

WOS: 000445937400016Purpose: The aim of this study is to evaluate the prognostic value and expression pattern of lamin A/C, lamin B1 and B2 in childhood acute myeloid leukemias, which are thought to be related with cell proliferation and apoptosis. Materials and Methods: Twenty-five patients diagnosed with acute myeloid leukemia (AML) and 35 control cases were included in the study. Real Time-Polymerase Chain Reaction (RT-PCR) method was used to detect lamin A/C, lamin B1 and lamin B2 protein gene expression at the time of the diagnosis and at the end of the induction treatment. Results: At the time of the diagnosis, lamin B1 protein gene expression was lower in AML patients when compared to the control group. When lamin protein expression levels at the time of diagnosis and after induction therapy were compared, the lamin A/C and B2 protein gene expressions were lower after the administration of induction chemotherapy. AML patients with hepatomegaly when compared to patients without hepatomegaly, had only increased lamin A/C protein gene expression. Patients with splenomegaly had both increased lamin A/C and lamin B2 protein gene expressions when compared to the patients without splenomegaly. Conclusion: In childhood acute myeloid leukemias, lamin B1 protein gene expression could be used as a diagnostic marker, while decrease in the lamin A/C and B2 protein gene expressions after induction could be used as a marker for response to therapy