Comparison of the effects of all-trans retinoic acid, methotrexate, actinomycin D, and combined chemotherapy on different choriocarcinoma cell culture models

WOS: 000455062500004Introduction: Our objective was to investigate the efficacy of all-trans retinoic acid (ATRA) alone and in combination chemotherapy with methotrexate (MTX) and combined with actinomycin D (Act-D) in choriocarcinoma cell culture models (JAR, JEG-3). Material and Methods: JAR and JEG-3 cells were cultured. ATRA, MTX and Act-D trial groups were determined with different doses. DMSO was applied as control group. Drugs were administered to the cells simultaneously, and 72 hours after drug administration, the cells were detached using trypsin-ethylenediamine tetraacetic acid solution. The degree of apoptosis was determined by flow cytometry. Statistical analyses of the apoptotic ratios were performed using SPSS 19.0 and the Wilcoxon test. Results: The ratio of apoptosis was statistically significant when only ATRA was applied on JAR and JEG-3 cell culture lines versus control group, p<0.05. The ratio of apoptosis was increased on JAR and JEG-3 cell culture lines, when ATRA was added in the combination of MTX 2 mu M, ACT-D 0.1 mu M, p