Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen

Objective: Cupressaceae pollen has commonly been reported to be an important aeroallergen and causal factor of spring, autumn and winter pollinosis in many countries. The aim of this study was to compare of the structure and allergenic protein content of Cupressus arizonica Greene., Cupressus sempervirens L. and Juniperus oxycedrus L. pollen in detail and contribute to Cupressaceae pollen allergen diagnosis and therapy studies in Turkey. Methods: The pollen structure were examined by LM and SEM. Pollen protein content was investigated by Bradford protein assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot analysis and two-dimensional polyacrylamide gel electrophoresis (2DE PAGE), respectively. Pectate lyase (PL) enzyme activities were compared. Immunoblotting was carried out by using extracts of the three taxa pollen collected from Turkey. Results: All three taxa was found very similar in terms of pollen morphology however, intine thickness was prominently different. Cupressus arizonica pollen extracts showed the lowest PL activity. Five sera specific IgE of all allergic subjects showed reaction with only C. arizonica pollen extracts. Conclusions: As a conclusion, the pollen structure, protein function or protein structure and isoforms of allergens could affects allergenic properties of the pollen. This study also may help to improve the Cupressaceae pollen allergen diagnosis and therapy. © 2018 Turkish Biochemistry Society. All rights reserved.2013/26Acknowledgments: This study was funded BAP Research Council (Project no: 2013/26) in Kırıkkale University. The author thanks Zeynep Mısırlıgil for technical assistance and the Department of Immunology and Allergy Diseases, Ankara University School of Medicine, for giving access to patient sera and data. The authors also thanks to TUBI-TAK MAM Genetic Engineering and Biotechnology Institute, Kocaeli, Turkey for providing antibodies