Comparison of Spermac and Diff-quik staining methods in the assessment of sperm morphology in asthenozoopermia cases [Asthenozoospermia olgularinda sperm morfolojisi degerlendirmede Spermac ve Diff-quik boya yöntemlerinin karşilaştirilmasi]

Sperm motility problems (Asthenozoopermia) is one of the major causes of male infertility. In cases with asthenozoopermia infertility increases to a greater extent when sperm morphology disorders are added to coexisting motility disorders. A total of 65 males (21 males with sperm motility disorder and 44 males without this disorder) who referred to the in vitro fertilization unit of Dokuz Eylul University Medical School between November 15, 2004 and April 5, 2005 were included in the present study. Spermac and Diff-quik staining methods were used in the assessment of sperm morphology in all semen samples. Test results showed consistency with both staining methods on 55 semen samples examined. Sperm morphology was normal in 32 semen samples with consistent test results by both methods, while the percentage of morphology was low in 23 cases. In 7 of remaining 10 cases, the sperm morphology was in the normal range with Diff-quik staining method while an abnormal percentage was detected with Spermac method. In 3 cases the sperm morphology was within normal limits with Spermac staining method while Diff-quik staining method demonstrated abnormal sperm morphology. Statistical analysis with Mc Nemar test demonstrated that both staining methods were reliable. Since neither of the two methods used in the evaluation of sperm morphology has proven superiority to the other, a cost reduction may be provided by choosing the economical one. © Gülhane Askeri Tip Akademisi 2008