Determination of volatile compounds from rosemary, sage, oregano and thyme after encapsulation : bachelor thesis

Brojna su istraživanja potvrdila korisno djelovanje ružmarina, kadulje, origana i timijana na ljudski organizam. Njihovo djelovanje potječe od bogatog sastava biološki aktivnih spojeva. Cilj ovoga rada bio je odrediti sastav hlapivih komponenti navedenih biljaka nakon postupka inkapsulacije koristeći dva nosača (protein sirutke i protein soje). Korištena tehnika za određivanje hlapivih komponenti bila je plinska kromatografija vezana uz masenu spektrometriju s mikroekstrakcijom na čvrstoj fazi, GC-MS-SPME a za određivanje ukupnih fenola koristila se spektrofotometrijska metoda Folin-Ciocalteu. Dobiveni rezultati pokazuju da se kemijski sastav hlapljivih komponenti navedenih biljaka razlikuje s obzirom na vrstu proteinskog nosača.Many studies have confirmed the positive effect of rosemary, sage, oregano and thym on the human organism. Their activity stems from the rich composition of biologically active compounds. The main purpose of this work was determination of the composition of the volatile components of the above mentioned plants after encapsulation using two carriers (whey proteins and soy proteins). The technique used to determine the volatile components was gas chromatography related to mass spectrometry with solid phase microextraction GC-MS-SPME, but for the determination of total phenol a spectrophotometric method of Folin-Ciocalteu was used. The results show that the chemical composition of the volatile components of mentioned plants varies with the type of protein carrier