In vitro multiplication performance of lysimachia nummularia L. under salinity stress

WOS:000503915900015Salinity levels are a serious abiotic stress factor affecting the metabolic and physiological activities of plants. Therefore, it is very important to know the responses of plants against salt. In this study, the effects of different NaCl concentrations (0-100 mM) on the growth of Lysimachia nummularia L. by tissue culture techniques were investigated. In addition, 0.25 mg/L 6-benzylaminopurine (BAP), Kinetin (KIN) and Thidiazuron (TDZ) were added to Murashige and Skoog (MS) nutrient media and the interactions of NaCl toxicity and hormones were evaluated. With the NaCl toxicity, yellowing and browning were observed in the leaves of the regenerated shoots. Regeneration of shoots on the explants was adversely affected by an increase in NaCl concentrations in the culture medium. In the culture medium with BAP, KIN, and TDZ, the maximum number of shoots per explant was recorded as 3.66, 4.16, 11.22 shoots/explant at 25 mM NaCl application, respectively. In BAP (4.27 cm) and TDZ (1.49 cm) culture medium, the highest shoot lengths were obtained in the control group and in the culture medium added with KIN (4.73 cm) was obtained in 25 mM NaCl application. Minimum shoot numbers and shoot lengths were determined in 100 mM NaCl application in three hormone species. This paper is a comprehensive study of the effects of salt stress on in vitro plant production and development