Pepsin extraction from bovine stomach using aqueous two-phase systems: Molecular mechanism and influence of homogenate mass and phase volume ratio
- Imelio, Natalia
- Marini, Analia Laura
- Spelzini, Darío
- Picó, Guillermo Alfredo
- Farruggia, Beatriz Monica
DOIAbstract
Pepsin partitioning, a gastric acid protease, in aqueous two-phase systems of polyethyleneglycol/potassium phosphate, sodium citrate and ammonium sulphate was assayed using polyethylenglycol of different molecular mass. Pepsin was found to be partitioned towards the polymer-rich phase in all the systems, which suggests an important protein-polymer interaction due to the highly hydrophobic character of the protein surface exposed to the solvent. The pepsin partitioning behavior was explained according to Timasheff's preferential interaction theory. The process was driven entropically with participation of structured water around the polyethyleneglycol ethylenic chains. The best pepsin recovery was observed in the systems polyethyleneglycol m...
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