Survival kinase-dependent pathways contribute to gender difference in the response to myocardial ischemia–reperfusion and ischemic post-conditioning
- Ciocci Pardo, Alejandro
- Scuriatti, Luis Emiliano
- González Arbeláez, Luisa Fernanda
- Caldiz, Claudia Irma
- Fantinelli, Juliana Catalina
- Mosca, Susana Maria
DOIAbstract
The response to ischemia/reperfusion and the effects of ischemic post-conditioning (IPC) are sex-dependent, but the mechanisms have not been clarified. Male (M) and female (F) rat hearts isolated and perfused using the Langendorff technique were subject to 30 min of global ischemia (GI) and 60 min reperfusion (R). In IPC hearts, three cycles of 30-sec GI/30-sec R were applied at the beginning of R. Infarct size and myocardial function were assessed. Superoxide production, antioxidant systems, and expressions of phosphorylated forms of serine/threonine kinase (Akt), glycogen synthase kinase 3β (GSK-3β), protein kinase C ε (PKCε), endothelial nitric oxide synthase (eNOS), and apoptosis were measured. In the basal state, superoxide production ...
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