LPL protein in Chronic Lymphocytic Leukaemia have different origins in Mutated and Unmutated patients. Advances for a new prognostic marker in CLL

Lipoprotein lipase (LPL) mRNA expression in chronic lymphocytic leukaemia (CLL) is associated with an unmutated immunoglobulin profile and poor clinical outcome. We evaluated the subcellular localization of LPL protein in CLL cells that did or did not express LPL mRNA. Our results show that LPL protein is differently located in CLL cells depending on whether it is incorporated from the extracellular medium in mutated CLL or generated de novo by leukaemic cells of unmutated patients. The specific quantification of endogenous LPL protein correlates with mRNA expression levels and mutational IGHV status, suggesting LPL protein as a possible reliable prognostic marker in CLL.Fil: Prieto, Daniel. Instituto Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas "Clemente Estable"; UruguayFil: Seija, Noé. Instituto Pasteur de Montevideo; Uruguay. Universidad de la República; UruguayFil: Uriepero, Angimar. Instituto Pasteur de Montevideo; UruguayFil: Souto-Padron, Thais. Universidade Federal do Rio de Janeiro; BrasilFil: Oliver, Carolina. Universidad de la República; UruguayFil: Irigoin, Victoria. Universidad de la República; UruguayFil: Guillermo, Cecilia. Universidad de la República; UruguayFil: Navarrete, Marcelo A.. Universidad de Magallanes; ChileFil: Inés Landoni, Ana. Ministerio de Salud. Administración Servicios de Salud del Estado. Hospital Maciel; UruguayFil: Dighiero, Guillermo. Ministerio de Salud. Administración Servicios de Salud del Estado. Hospital Maciel; UruguayFil: Gabus, Raúl. Ministerio de Salud. Administración Servicios de Salud del Estado. Hospital Maciel; UruguayFil: Giordano, Mirta Nilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Oppezzo, Pablo. Instituto Pasteur de Montevideo; Urugua