Postharvest ozone fumigation of Petit Verdot grapes to prevent the use of sulfites and to increase anthocyanin in wine.

Background andAims: The request by the consumer for safer food is also pushing thewine sector to find alternative solutions to sulfur dioxide. The aim of this research has been to evaluate the application of ozone as a sanitising agent of grapes before vinification in order to avoid the use of sulfur dioxide. Methods and Results: Postharvest ozone fumigation overnight of Petit Verdot grapes increased anthocyanin concentration by 19%versus 9%in the Control (untreated) during fermentation/maceration. Fermentation kinetics were faster in winemade from ozone-treated grapes (16 vs 21 days for the Control), and extraction of phenolic substances and anthocyanin wasmore rapid than that in the Control wines, Ozone treatment significantly reduced the count of Saccharomyces cerevisiae and non-Saccharomyces yeasts but also significantly decreased the acetic acid bacteria. The final wine produced from ozone-treated grapes was characterised by low volatile acidity (similar to that of the Control wine) with a significant reduction in sulfur dioxide (17 and 8mg/L, respectively, total and free sulfur dioxide). Sensory evaluation revealed a strong fruity aroma. Conclusions: Ozone gas treatment of grapes reduced the microbial count significantly and increased the extraction of phenolic substances and the aroma of the final wine. Significance of the Study: Postharvest ozone fumigation can be used to produce wine without sulfur dioxid