Uloga centrifugirane tekućinske citologije u procjeni endometrija kobila

Endometritis is frequently encountered in brood mare practice and is one of the main causes of infertility. Several methods, including among others cytology, bacteriology and histopathology, are used for diagnosis, however, even the combined use of these methods may not produce a definitive result. The objective of this study was to make an assessment of the diagnostic efficiency of centrifuged liquid-based cytology (LBC) in comparison to the determination of: 1) the presence of polymorphonuclear cells (PMNs) in the uterine epithelium and stratum compactum by histopathology; 2) the presence of PMNs by cytology and 3) the presence of uterine infection by microbiology. Fifty mares of varying age, which belonged to the Jockey Club of Turkey, were used in this study. Endometrial samples were harvested using the cytobrush (CB) and endometrial biopsy (EB) techniques for microbiological, Cytological and histopathological examinations. The comparison of liquid- based cytology (LBC) with histopathological diagnosis (HD), which is considered to be the best standard, showed that the sensitivity (SE), specificity (SP), predictive value of positive test (PVP), and predictive value of negative test (PVN) of LBC were 0.5000, 0.4000, 0.7692 and 0.6087, respectively. No coherence was detected between the results of these two methods (P = 0.0166). Furthermore, it was ascertained that LBC failed in detecting degenerative and fibrotic alterations in the endometrial glands. LBC slides provide sharp images of well-dispersed cells, and enable greater diagnostic sensitivity and certainty. Thus, as the area examined is small, the identification of the target cells is relatively easy.Endometritis je čest u rasplodnih kobila i jedan je od glavnih uzroka njihove neplodnosti. Za dijagnostiku ovoga stanja primjenjuje se nekoliko metoda, uključujući citološke, bakteriološke i histopatološke metode, no čak ni njihova kombinirana primjena ne mora dati konačan rezultat. Cilj je ovoga istraživanja bio procijeniti dijagnostičku učinkovitost centrifugirane tekućinske citologije (LBC) kako bi se usporedila prisutnost polimorfonuklearnih stanica (PMN) u materničnom epitelu i vanjskom sloju endometrija (stratum compactum): 1. histopatološkim metodama, 2. citologijom, 3. mikrobiološki (s obzirom na prisutnost uzročnika upale). U istraživanje je uključeno pedeset kobila različite dobi, uzgojenih u konjičkom klubu Jockey Club of Turkey. Uzorci endometrija za mikrobiološku, citološku i histopatološku pretragu dobiveni su CB i EB tehnikama. Usporedba tekućinske citologije (LBC) s patohistološkom dijagnostikom (HD), koja se smatra najboljom metodom, pokazala je da su osjetljivost, specifičnost i pozitivna prediktivna vrijednost testa (PVP) te negativna prediktivna vrijednost testa (PVN) tekućinskom citometrijom bile 0,5000, 0,4000, 0,7692 i 0,6087. Rezultati ovih dviju metoda nisu se podudarali (P = 0,0166). Nadalje, potvrđeno je da LBC nije pouzdana u otkrivanju degenerativnih i fibroznih promjena u žlijezdama endometrija. U pozitivnom smilu LBC pruža jasnu sliku dobro disperziranih stanica te omogućuje veću dijagnostičku osjetljivost i pouzdanost. Također, kako je ispitivano područje malo, identifikacija ciljanih stanica relativno je lagana