Spatial ecology of loggerhead turtles: Insights from stable isotope markers and satellite telemetry

This is the final version. Available on open access from Wiley via the DOI in this recordAim Using a combination of satellite telemetry and stable isotope analysis (SIA), our aim was to identify foraging grounds of loggerhead turtles (Caretta caretta) at important rookeries in the Mediterranean, examine foraging ground fidelity, and across 25 years determine the proportion of nesting females recruiting from each foraging region to a major rookery in Cyprus. Location Mediterranean Sea. Methods Between 1993 and 2018, we investigated the spatial ecology of loggerhead turtles from rookeries in Cyprus and Greece using satellite telemetry (n = 55 adults) and SIA of three elements (n = 296). Results Satellite telemetry from both rookeries revealed the main foraging areas as the Adriatic region (Cyprus: 4% of individuals, Greece: 55%), Tunisian Plateau (Cyprus: 16%, Greece: 40%) and the eastern Mediterranean (Cyprus: 80%, Greece: 5%). Combining satellite telemetry and SIA allowed 64% of all nesting females to be assigned to; the Adriatic region (Cyprus: 2%, Greece: 38.5%), Tunisian Plateau (Cyprus: 47%, Greece: 38.5%) and the eastern Mediterranean (Cyprus: 51%, Greece: 23%), which are markedly different to proportions obtained using satellite telemetry. The proportion of the Cyprus nesting cohort using each foraging region did not change significantly, with the exception that individuals foraging in the Adriatic region are only present in the Cyprus nesting population from 2012. Repeat satellite tracking (n = 3) and temporal consistency in isotope ratios (n = 36) of Cyprus females, strongly suggest foraging ground fidelity over multiple decades. Main conclusions This study demonstrates the advantages of combining satellite telemetry and SIA to investigate spatial ecology at a population level. The importance of the Tunisian Plateau for foraging is demonstrated. This study indicates that females generally show high fidelity to foraging grounds and shows a potential recent shift to foraging in the Adriatic region for Cyprus females, while the importance of other regions persists across decades, thus providing baselines to develop and assess conservation strategies.Natural Environment Research Council (NERC