IDENTIFICATION OF GLYCONE TYPES IN THE CROWN FLOWER OF BATU LOCAL ROSES USING LC-MS ANALYSIS

Rose flower is symbol of love that has plenty of benefits. It can be used for preventing and treating various diseases. Rose is known to contain anthocyanin pigments in which a glycone compound is bonded to aglycone as its anthocyanidine. Anthocyanins of Batu local rose were characterized as cyanidin and malvidin-glycosides. This study aimed to identify the type of glycone contained in the crown of roses using LC-MS (Liquid Chromatography Mass Spectrometry) analysis. Research was conducted by observing changes in the content of anthocyanin pigment of roses stored for few days and by identifying the type of glycone contained in the crown of roses. Selected local roses from Punten, District of Sidomulyo, Batu City, Indonesia were used to improve the usability of these widely cultivated roses. Anthocyanin pigment isolates were obtained from C18 column chromatographic fractionation (Shepadex G25 as stationary phase, Shimadzu). Prior to isolation, anthocyanin pigments were extracted using aquadest and lactic acid (with ethanol, HCl, BAA, and BuOH-HCl as developer materials), then were analyzed using a TLC plate. LC-MS analyses in molecular weights indicated that the crown of roses contained six types of glycone namely maltose (180), glucose (162), rhamnose (146), acids coumaril (146), xylose (132), and rutinose (308) m/z