Synthesis and Purification of Oligonucleotide N3′→︀P5′ Phosphoramidates and their Phosphodiester and Phosphorothioate Chimeras

This unit describes the synthesis and purification of oligonucleotide N3′→︀P5′ phosphoramidates, wherein each 3′‐oxygen is replaced by a 3′‐amine in the 2′‐deoxyribose ring. The synthesis of required monomers and application of the method to preparation of phosphodiester‐ and phosphorothioate‐containing chimera of phosphoramidate is also reported.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/153159/1/cpnc0407.pd