Profile Antibiotics Resistance On Escherichia Coli Isolated From Raw Milk In Surabaya Dairy Farms, Indonesia

Aim : The study was undertaken to isolate pathogenic E. coli from raw milk in Surabaya area and investigate their antibiotic sensitivity pattern. Materials and Methods: During 4 months duration of study a total of 128 raw milk samples were collected from different places of dairy farms located in Surabaya, under aseptic precautions. For the enrichment of the organism from the collected samples, MacConkey broth was used and inoculation was carried out on MacConkey agar and EMB agar, to confirm the isolates, various biochemical tests such as IMViC test were performed. Antibiotic sensitivity pattern of E.coli to antibiotic agents was evaluated by disk diffusion method. Results: The result of present study revealed that out of 128 samples, 86 samples were found contaminated with E.coli. Antibiotic sensitivity pattern revealed high resistance against ampicillin (95.3 %), whereas moderate resistance was observed for chloramphenicol (39.5 %). Also sensitive antibiotic was observed for ciprofloxacin, gentamicin and trimethoprim. Conclusion: Current study supports the finding that raw milk can be regarded as critical source of pathogenic E. coli and pose potential public health hazard