Prospektif Kombinasi Susu Probiotik Lactobacillus acidophilus dan Bifidobacterium bifidum Sebagai Sediaan Anti Diare

In the course of our screening program for anti diarrheal bacterial – Escheria coli, Salmonella typhimurium, and Vibrio cholerae agents from fermented probiotic milk, Lactobacillus acidophilus FNCC-0051 and Bifidobacterium bifidum as a potential probiotic were picked out for further investigation. Each probiotic microorganism was prepared as fermented milk by using 15% skim milk. Inhibition potency of the fermented milk that consisted of both probiotic microorganisms at 1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, and 9:1 ratio was assayed against diarrheal causing bacteria. The combination of two fermented probiotic milk showed strong inhibition against test bacteria at ratio of 4:6 and the MIC is 30%. On the other hands, total plate count of the combination is 108 cfu/mL, is comply with the requirement for intestine as target side of action. Thus, the combination was considered of great potential to develop as an anti diarrheal preparation