Automated differential fluorometric analysis of norepinephrine and epinephrine in blood plasma and urine.

OAM report.PDFTech ReportFAA-AM-71-15DOT/FAA/AM-71/15Aviation medicineBlood analysisUrineFluorometersStress (Physiology)Fatigue (Physiological condition)ChromatographyChemical analysisSolutions (Chemistry)MixturesReagentsDistillationCentrifugal forceElectromagnetic waves by wavelengthLightHydrogenSodiumOxygenChlorineAcetic acidUreaWaterIron compoundsHydroxidesChlorine compoundsCarbonNitrogenChemical compoundsInorganic compoundsOrganic compoundsAminesOxidationMeasurementStandard deviationHuman subject testingUnited StatesCivil Aerospace Medical InstituteFiorica, VincentMoses, RussellCivil Aeromedical InstituteUnited States. Office of Aviation MedicineNTL-AVIATION-AVIATIONNTL-AVIATION-Aviation Human FactorsUS Transportation CollectionAn automated fluorometric trihydroxyindole procedure is described for the measurement of norepinephrine (NE) and epinephrine (E) in blood plasma or urine. The method employs conventional techniques for isolation of the catecholamines by alumina column chromatography. Column eluates are analyzed in an AutoAnalyzer system incorporating two fluorometers. Differentiation of the amines is based on differences in the fluorescence characteristics of the lutines of NE and E.The accuracy (as estimated by recoveries of added amines) and precision of the method are comparable to those reported for other trihydroxyindole techniques. Representative values for urinary excretion and for plasma levels of NE and E compare favorably with values stated in the literature