Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
- Stege, Patricia Wanda
- Messina, Germán Alejandro
- Sombra, Lorena Luján
- Bianchi, Guillermo
- Olsina, Roberto Antonio
DOIAbstract
Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10-4 and 6.4 × 10-4 mg mL-1, respecti...
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