Prolactin (PRL) induction of cyclooxygenase 2 (COX2) expression and prostaglandin (PG) production in hamster Leydig cells
- Matzkin, Maria Eugenia
- Ambao, Veronica Ana
- Carino, Mónica Herminia
- Rossi, Soledad Paola
- Gonzalez, Lorena
- Turyn, Daniel
- Campo, Stella Maris
- Calandra, Ricardo Saul
- Frungieri, Monica Beatriz
DOIAbstract
Serum prolactin (PRL) variations play a crucial role in the photoperiodic-induced testicular regression–recrudescence transition in hamsters. We have previously shown that cyclooxygenase 2 (COX2), a key enzyme in the biosynthesis of prostaglandins (PGs), is expressed mostly in Leydig cells of reproductively active hamsters with considerable circulating and pituitary levels of PRL. In this study, we describe a stimulatory effect of PRL on COX2/PGs in hamster Leydig cells, which is mediated by IL-1β and prevented by P38-MAPK and JAK2 inhibitors. Furthermore, by preparative isoelectric focusing (IEF), we isolated PRL charge analogues from pituitaries of active [isoelectric points (pI): 5.16, 4.61, and 4.34] and regressed (pI: 5.44) hamsters. M...
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