Polymorphism Diacylglycerol Acyltransferase 1 (DGAT1) Gene in Bali Cattle

Bali cattle is a native cattle from Indonesia that has tremendous potential to be developed. However, the utilization is not yet optimal. Carcass quality is one of the unoptimized traits and molecular approach is used to study this trait. One of the markers that can be used to study carcass quality is Diacylglycerol Acyltransferase 1 (DGAT) gene. The purpose of this study was to identify the diversity of Diacylglycerol Acyltransferase 1 (DGAT) gene in Bali cattle at Bali Cattle Breeding Center. Sequencing method and defining the right SNP on the target fragment of DGAT1 gene were used for this study. The number of samples used in this study were 48 heads of Bali cattle consisting of 24 males and 24 females. Primer DGAT1 gene amplification used forward 5'-CACCATCCTCTTCCTCA-3’ and reverse 5'-GGAAGCGCTTTCGGATG-3'. Identification of DGAT1 gene diversity was conducted by direct sequencing of allele and genotype methods. Hardy-Weinberg balance was calculated by GENEPOP (V3.2). The result of analysis found SNP in DGAT1 gene in Bali cattle. There were SNP c.10433G A and c.10434C A, but they were monomorphic